Coding

Part:BBa_K4060508:Design

Designed by: Cheng-Ju Lu   Group: iGEM21_NYCU-Taipei   (2021-10-21)


QPAS1_mCherry fusion protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 454
    Illegal SpeI site found at 490
    Illegal PstI site found at 847
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 454
    Illegal SpeI site found at 490
    Illegal PstI site found at 847
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 454
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 454
    Illegal SpeI site found at 490
    Illegal PstI site found at 847
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 454
    Illegal SpeI site found at 490
    Illegal PstI site found at 847
    Illegal NgoMIV site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The construction of this part is according to igem assembly protocol.


Source

Both QPAS1 gene and mCherry gene are cloned from Addgene plasmid pKA-207I10

References